Infection mechanisms of a bacteriophage targeting motile bacteria
Bacteriophages are viruses of bacteria that use the host metabolism for propagation of new phage particles. One unique group of bacteriophages, named flagellotropic phages, are targeting the bacterial flagellum and rotation of the flagellar filament is an essential requirement for infection. Flagellar-driven motility and chemotaxis enables motile bacteria to move away from harmful and towards beneficial chemicals. The soil bacterium Agrobacterium sp. H13-3 is the specific host for the flagellotropic phage 7-7-1. Co-evolution of bacteria and their phages resulted in the development of species-specific phage-host interactions. Previous research by the Scharf lab discovered that the flagellum and lipopolysaccharides (LPS) of A. sp. H13-3 serve as essential primary and secondary surface receptors for phage 7-7-1. However, nothing is known about the mechanisms of phage adsorption to the flagellum, translocation along the flagellum, or the subsequent infection steps. The overarching goal of this project is to characterize the specific processes involved in flagellotropic phage infection and host adaptation.
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