Sinorhizobium meliloti motility and chemotaxis

R.C. Sobe & Scharf B.E. (2024) “The swimming defect caused by absence of the transcriptional regulator LdtR in Sinorhizobium meliloti is restored by mutations in the motility genes motA and motS” Molecular Microbiology; 121(5):954-970.

R.C. Sobe, Gilbert C., Vo L., Alexandre G., Scharf B.E. (2022) FliL and its paralog MotF have distinct roles in the stator activity of the Sinorhizobium meliloti flagellar motor. Molecular Microbiology 118(3), 223-243.

M.A.B. Kreutzberger, Sonani R.R., Liu J., Chatterjee S., Wang F., Sebastian A.L., Biswas P., Ewing C., Zheng W., Poly F., Frankel G., Luisi B.F., Calladine C.R., Krupovic M., Scharf B.E., Egelman E.H. (2022) Convergent evolution in the supercoiling of prokaryotic flagellar filaments. Cell 15;185(19):3487-3500.

S. Salar, Ball N.E., Baaziz H., Nix J.C., Sobe R.C., Compton K.K., Zhulin I.B., Brown A.M., Scharf B.E., Schubot F.D. (2023) The structural analysis of the periplasmic domain of Sinorhizobium meliloti chemoreceptor McpZ reveals a novel fold and suggests a complex mechanism of transmembrane signaling. Proteins: Structure, Function, and Bioinformatics.; 91(10):1394-1406.

H. Baaziz, K.K. Compton, Hildreth S.B., Helm R.F., Scharf B.E. (2021) McpT, a broad range carboxylate chemoreceptor in Sinorhizobium meliloti. Journal of Bacteriology, 203(17), 1-16.

T.D. Arapov, Castañeda Saldaña R., Sebastian A.L., Ray W.K., Helm R.F., Scharf B.E. (2020) Cellular stoichiometry of chemotaxis proteins in Sinorhizobium meliloti. Journal of Bacteriology; 202(14), 141-120.

K.K. Compton, Hildreth S.B., Helm R.F., Scharf B.E. (2018) The Sinorhizobium meliloti chemoreceptor McpV senses short chain carboxylates via direct binding; Journal of Bacteriology 200(23), 1-16.

 M. Shrestha, Compton K.K., Mancl J., Webb B.A., Scharf B.E., Schubot, F.D. (2018) Crystal structure of the sensory domain of the first known bacterial betaine chemosensor: The methyl-accepting chemotaxis protein X (McpX) from Sinorhizobium meliloti. Biochemical Journal 14; 475(24), 3949-3962.

H.M. Zatakia, Arapov T.D., Meier V.M., Scharf B.E. (2018) Cellular stoichiometry of methyl-accepting chemotaxis proteins in Sinorhizobium meliloti. Journal of Bacteriology, 200(6). (PMID: 29263102)

B.A. Webb, Compton, K.K., Martin del Campo J.S., Taylor D., Sobrado P., Scharf B.E. (2017) Sinorhizobium meliloti chemotaxis to multiple amino acids is mediated by chemoreceptor McpU. Molecular Plant Microbe Interactions 30 (10), 770-777. (DOI: 10.1094/MPMI-04-17-0096-R)

B.A. Webb, Compton K.K., Saldaña R.C., Arapov T., Ray W.K., Helm R.F., Scharf B.E. (2017) Sinorhizobium meliloti chemotaxis to quaternary ammonium compounds is mediated by the chemoreceptor McpX. Molecular Microbiology 103(2), 333-346. (DOI: 10.1111/mmi.13561)

B.A. Webb, Helm R.F., Scharf B.E. (2016) Contribution of individual chemoreceptors to Sinorhizobium meliloti chemotaxis towards amino acids of host and non-host seed exudates. Molecular Plant Microbe Interactions 29(3), 231-239. (DOI:10.1094/MPMI-12-15-0264-R)

Amin, Kothamachu V., Feliu E., Scharf B.E., Porter S.L., Soyer O.S. (2014) Phosphate sink containing two-component signaling systems as tunable threshold devices. PLOS Computational Biology 10(10), 1-11. (DOI:10.1371/journal.pcbi.1003890)

Webb, Hildreth S., Helm R.F., Scharf B.E. (2014) The Sinorhizobium meliloti chemoreceptor McpU directly binds proline to mediate chemotaxis towards host plant exudates. Applied & Environmental Microbiology 80(11), 3404-3415. (DOI:10.1128/AEM.00115-14)

H.M. Zatakia, Nelson C.E., Syed U.J., Scharf B.E. (2014) ExpR coordinates the expression of symbiotically important, bundle-forming Flp pili with quorum sensing in Sinorhizobium meliloti. Applied & Environmental Microbiology 80(8), 2429-2439. (DOI:10.1128/AEM.04088-13)

D. Wibberg, Blom J., Rückert C., Winkler A., Albersmeier A., Pühler A., Schlüter A., Scharf B.E (2013) Draft genome sequence of Sinorhizobium meliloti RU11/001, a model organism for flagellum structure, motility and chemotaxis. Journal of Biotechnology 168(4), 731-733. (DOI:10.1016/j.jbiotec.2013.10.015)

D. Dogra, Purschke F.G., Wagner V., Haslbeck M., Kriehuber T., Hughes J.G., Van Tassell M.L., Gilbert C., Niemeyer M., Ray W.K., Helm R.F., Scharf B.E. (2012) Sinorhizobium meliloti CheA complexed with CheS exhibits enhanced binding to CheY1 resulting in accelerated CheY1-P dephosphorylation. Journal of Bacteriology 194, 1075-1087. [cited in faculty of 1000] (DOI:10.1128/JB.06505-11)

V.M. Meier and B.E. Scharf (2009) Cellular localization of predicted transmembrane and soluble chemoreceptors in Sinorhizobium meliloti. Journal of Bacteriology 191, 5724-33. (DOI:10.1128/JB.01286-08)

Riepl, Maurer T., Kalbitzer H.R., Meier V.M., Schmitt R., Scharf B. (2008) Interaction of CheY2 and CheY2-P with the cognate CheA kinase in the chemosensory-signalling chain of Sinorhizobium meliloti. Molecular Microbiology 69, 1373-1384. (DOI:10.1111/j.1365-2958.2008.06342.x)

V.M. Meier, Muschler P., Scharf B.E. (2007) Functional analysis of nine putative chemoreceptor proteins in Sinorhizobium meliloti. Journal of Bacteriology 189, 1816-1826. (DOI:10.1128/JB.00883-06)

Rotter, Mühlbauer S., Schmitt R., Scharf B. (2006) Rem, a new transcriptional regulator of the flagellar regulon in Sinorhizobium meliloti. Journal of Bacteriology 188, 6932-6942. (DOI:10.1128/JB.01902-05)

Eggenhofer, Rachel R., Haslbeck M., Scharf B. (2006) MotD of Sinorhizobium meliloti and related a-proteobacteria is the flagellar hook-length regulator and therefore re-assigned as FliK. Journal of Bacteriology 188, 2144-2153. (DOI:10.1128/JB.188.6.2144-2153.2006)

Attmannspacher, Scharf B., Schmitt R. (2005) Control of speed modulation (chemokinesis) in the unidirectional rotary motor of Sinorhizobium meliloti. Molecular Microbiology 56, 708-718.  (DOI:10.1111/j.1365-2958.2005.04565.x)

Eggenhofer, Haslbeck M., Scharf B. (2004) The novel protein MotE serves as chaperone for the periplasmic motor protein MotC in Sinorhizobium meliloti. Molecular Microbiology 52, 701-712.DOI:10.1111/j.1365-2958.2004.04022.x)

Riepl, Scharf B., Schmitt R., Maurer T., Kalbitzer H.R. (2004) Solution structures of the inactive and BeF3-activated response regulator CheY2. Journal of Molecular Biology 338, 287-297. [cited in faculty of 1000] (DOI:10.1016/j.jmb.2004.02.054)

Scharf (2002) Real-time imaging of fluorescent flagellar filaments of Rhizobium lupini H13-3: Flagellar rotation and pH-induced polymorphic transitions. Journal of Bacteriology 184, 5979-5986. (PMCID:PMC135403)

Scharf, Schuster-Wolff-Bühring H., Rachel R., Schmitt R. (2001) Mutational analysis of the Rhizobium lupini H13-3 and Sinorhizobium meliloti flagellin genes: Importance of flagellin A for flagellar filament structure and transcriptional regulation. Journal of Bacteriology 183, 5334-5342. (DOI:10.1016/j.jmb.2004.02.054)PMCID:PMC135403